Tokai Hit stage top incubator
Last modified by mliljest@helsinki_fi on 2024/01/24 07:25
Incubation system with STXG controller unit and WSKMX stage top chamber. Temperature, humidity and CO2 control.
Suggested amount of dH2O in water bath: 32ml
Temperature set points:
S/N | 35mm dish | Well plate |
|---|---|---|
Top | 43.0 °C | 43.5 °C |
Bath | 41.0 °C | 41.0 °C |
Stage | 38.0 °C | 37.5 °C |
Lens heater set values according to objective (WD, Imm) and dish bottom material:
Magnification | Objective | NA | Imm. | Glass bottom | Plastic bottom |
5x | N Plan | 0.12 | Dry | OFF | OFF |
20x | HC PL Fluotar Corr | 0.4 | Dry | OFF | OFF |
20x | HC PL Apo | 0.8 | Dry | 36 °C | 39 °C |
40x | HC PL Fluotar | 0.8 | Dry | 36 °C | 39 °C |
40x | HC PL Apo CS2 | 1.3 | Oil | 41 °C | - |
All set values from instrument manual and have not been confirmed by BIU. Use your own discretion.