2022 Leica Thunder Imager 3D Cell Culture
Last modified by Elnaz Fazeli on 2024/01/31 11:20
On demo at the BIU in May, 2022
Location: Biomedicum 1, Room B115a2 (Nikon TIRF/STORM room)
Motorized Leica DMi8 inverted epifluorescence microscope with motorized stage.
- Leica Thunder computational clearing for 3D imaging
- Fast multichannel imaging
- LAS X Navigator for multipoint imaging and large area stitching
- AFC hardware autofocus
- Stage top incubator from Tokai Hit for live cell imaging available on request
https://www.leica-microsystems.com/products/thunder-imaging-systems/p/thunder-imager-3d-live-cell/
Technical Information and capabilities
Objectives
Magnification | Objective | NA | Phase | Imm. | WD | AFC | Pixel size | Theoretical lens x-y resolution at 550nm | Theoretical lens z resolution at 550nm |
5x | N Plan | 0.12 | Ph0 | Dry | 14.0mm | - | 1.3µm | 2.796µm | 38.194µm |
20x | HC PL Fluotar Corr | 0.4 | Ph1 | Dry | 7.5mm | + | 0.325µm | 0.839µm | 3.438µm |
20x | HC PL Apo | 0.8 | - | Dry | 0.4mm | + | 0.325µm | 0.419µm | 0.859µm |
40x | HC PL Fluotar | 0.8 | PH2 | Dry | 0.4mm | + | 0.162µm | 0.419µm | 0.859µm |
40x | HC PL Apo CS2 | 1.3 | - | Oil | 0.24mm | + | 0.162µm | 0.258µm | 0.494µm |
Filters
- Quad cube for imaging (DAPI, FITC, TRITC, Cy5) including filter wheel for excitation.
Camera
- Leica DFC9000GTC 4.2MPx sCMOS B&W camera.
Lightsource
- Lumencor Spectra X 7-line LED light source.
Software
- LAS X 3.7.4