Wiki source code of Super-resolution
Last modified by Jussi Kenkkilä on 2024/03/13 13:12
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1 | **Super-resolution imaging**(% style="color:#222222" %) is based on a set of techniques that enhance the (%%)spatial resolution(% style="color:#222222" %) of an (%%)imaging(% style="color:#222222" %) system beyond that of the conventional widefield and confocal microscopes. | ||
2 | |||
3 | {{toc maxLevel="1"/}} | ||
4 | |||
5 | = GE DeltaVision OMX SR = | ||
6 | |||
7 | (% class="wikigeneratedid" id="HRoomA123b" %) | ||
8 | **Room A123b** | ||
9 | |||
10 | (% class="wikigeneratedid" %) | ||
11 | **[[image:OMX_3_1920_1200.jpg||height="31%" width="50%"]]** | ||
12 | |||
13 | == Objectives == | ||
14 | |||
15 | (% class="wrapped" %) | ||
16 | |=(% style="text-align: center;" %)(% style="text-align: center;" %) | ||
17 | ((( | ||
18 | Type | ||
19 | )))|=(% style="text-align: center;" %)(% style="text-align: center;" %) | ||
20 | ((( | ||
21 | Magnification / NA | ||
22 | )))|=(% colspan="1" %)(% colspan="1" %) | ||
23 | ((( | ||
24 | Coverglass thickness (mm) | ||
25 | )))|=(% colspan="1" %)(% colspan="1" %) | ||
26 | ((( | ||
27 | Working distance (mm) | ||
28 | )))|=(% colspan="1" style="text-align: center;" %)(% colspan="1" style="text-align: center;" %) | ||
29 | ((( | ||
30 | Suggested use | ||
31 | ))) | ||
32 | |(% style="text-align:center" %)(% style="text-align: center;" %) | ||
33 | ((( | ||
34 | PlanApo N | ||
35 | )))|(% style="text-align:center" %)(% style="text-align: center;" %) | ||
36 | ((( | ||
37 | 60×/1.42 Oil | ||
38 | )))|(% colspan="1" %)(% colspan="1" %) | ||
39 | ((( | ||
40 | 0.17 | ||
41 | )))|(% colspan="1" %)(% colspan="1" %) | ||
42 | ((( | ||
43 | 0.15 | ||
44 | )))|(% colspan="1" style="text-align:center" %)(% colspan="1" style="text-align: center;" %) | ||
45 | ((( | ||
46 | SIM imaging | ||
47 | ))) | ||
48 | |(% style="text-align:center" %)(% style="text-align: center;" %) | ||
49 | ((( | ||
50 | Apo N | ||
51 | )))|(% style="text-align:center" %)(% style="text-align: center;" %) | ||
52 | ((( | ||
53 | 60×/1.49 Oil | ||
54 | )))|(% colspan="1" %)(% colspan="1" %) | ||
55 | ((( | ||
56 | 0.13 - 0.19 | ||
57 | )))|(% colspan="1" %)(% colspan="1" %) | ||
58 | ((( | ||
59 | 0.10 | ||
60 | )))|(% colspan="1" style="text-align:center" %)(% colspan="1" style="text-align: center;" %) | ||
61 | ((( | ||
62 | TIRF imaging | ||
63 | ))) | ||
64 | |(% style="text-align:center" %)(% style="text-align: center;" %) | ||
65 | ((( | ||
66 | UPlanSApo | ||
67 | )))|(% style="text-align:center" %)(% style="text-align: center;" %) | ||
68 | ((( | ||
69 | 60×/1.30 Si | ||
70 | )))|(% colspan="1" %)(% colspan="1" %) | ||
71 | ((( | ||
72 | 0.15 - 0.19 | ||
73 | )))|(% colspan="1" %)(% colspan="1" %) | ||
74 | ((( | ||
75 | 0.30 | ||
76 | )))|(% colspan="1" style="text-align:center" %)(% colspan="1" style="text-align: center;" %) | ||
77 | ((( | ||
78 | Widefield imaging | ||
79 | ))) | ||
80 | |||
81 | == Lasers == | ||
82 | |||
83 | 4 diode laser modules for SIM, TIRF and widefield imaging. | ||
84 | |||
85 | * 405 nn | ||
86 | * 488 nm | ||
87 | * 568 nm | ||
88 | * 640 nm | ||
89 | |||
90 | == Detectors == | ||
91 | |||
92 | * 3 sCMOS cameras | ||
93 | |||
94 | == Software == | ||
95 | |||
96 | ==== Image Acquisition ==== | ||
97 | |||
98 | AquireSR 4.4 | ||
99 | |||
100 | ==== Image Reconstruction ==== | ||
101 | |||
102 | SoftWoRx 7.0 | ||
103 | |||
104 | ==== **Test your own fluorophores on this optical system** ==== | ||
105 | |||
106 | To do so, click on the link below, choose the right configuration and add your own fluorophore. You will see the efficiency of the system for that specific label. | ||
107 | |||
108 | [[https:~~/~~/www.fpbase.org/microscope/o44EGQpjxhqwdcehWQukx/>>url:https://www.fpbase.org/microscope/o44EGQpjxhqwdcehWQukx/||shape="rect"]] |