Wiki source code of Super-resolution

Last modified by Jussi Kenkkilä on 2024/03/13 13:12
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Jussi Kenkkilä 15.1 1 **Super-resolution imaging**(% style="color:#222222" %) is based on a set of techniques that enhance the (%%)spatial resolution(% style="color:#222222" %) of an (%%)imaging(% style="color:#222222" %) system beyond that of the conventional widefield and confocal microscopes.
jkenkkil@helsinki_fi 12.1 2
3 {{toc maxLevel="1"/}}
4
5 = GE DeltaVision OMX SR =
6
Jussi Kenkkilä 16.1 7 (% class="wikigeneratedid" id="HRoomA123b" %)
8 **Room A123b**
jkenkkil@helsinki_fi 12.1 9
Jussi Kenkkilä 16.1 10 (% class="wikigeneratedid" %)
11 **[[image:OMX_3_1920_1200.jpg||height="31%" width="50%"]]**
12
jkenkkil@helsinki_fi 12.1 13 == Objectives ==
14
15 (% class="wrapped" %)
16 |=(% style="text-align: center;" %)(% style="text-align: center;" %)
17 (((
18 Type
19 )))|=(% style="text-align: center;" %)(% style="text-align: center;" %)
20 (((
21 Magnification / NA
22 )))|=(% colspan="1" %)(% colspan="1" %)
23 (((
24 Coverglass thickness (mm)
25 )))|=(% colspan="1" %)(% colspan="1" %)
26 (((
27 Working distance (mm)
28 )))|=(% colspan="1" style="text-align: center;" %)(% colspan="1" style="text-align: center;" %)
29 (((
30 Suggested use
31 )))
Jussi Kenkkilä 15.1 32 |(% style="text-align:center" %)(% style="text-align: center;" %)
jkenkkil@helsinki_fi 12.1 33 (((
34 PlanApo N
Jussi Kenkkilä 15.1 35 )))|(% style="text-align:center" %)(% style="text-align: center;" %)
jkenkkil@helsinki_fi 12.1 36 (((
37 60×/1.42 Oil
38 )))|(% colspan="1" %)(% colspan="1" %)
39 (((
40 0.17
41 )))|(% colspan="1" %)(% colspan="1" %)
42 (((
43 0.15
Jussi Kenkkilä 15.1 44 )))|(% colspan="1" style="text-align:center" %)(% colspan="1" style="text-align: center;" %)
jkenkkil@helsinki_fi 12.1 45 (((
46 SIM imaging
47 )))
Jussi Kenkkilä 15.1 48 |(% style="text-align:center" %)(% style="text-align: center;" %)
jkenkkil@helsinki_fi 12.1 49 (((
50 Apo N
Jussi Kenkkilä 15.1 51 )))|(% style="text-align:center" %)(% style="text-align: center;" %)
jkenkkil@helsinki_fi 12.1 52 (((
53 60×/1.49 Oil
54 )))|(% colspan="1" %)(% colspan="1" %)
55 (((
56 0.13 - 0.19
57 )))|(% colspan="1" %)(% colspan="1" %)
58 (((
59 0.10
Jussi Kenkkilä 15.1 60 )))|(% colspan="1" style="text-align:center" %)(% colspan="1" style="text-align: center;" %)
jkenkkil@helsinki_fi 12.1 61 (((
62 TIRF imaging
63 )))
Jussi Kenkkilä 15.1 64 |(% style="text-align:center" %)(% style="text-align: center;" %)
jkenkkil@helsinki_fi 12.1 65 (((
66 UPlanSApo
Jussi Kenkkilä 15.1 67 )))|(% style="text-align:center" %)(% style="text-align: center;" %)
jkenkkil@helsinki_fi 12.1 68 (((
69 60×/1.30 Si
70 )))|(% colspan="1" %)(% colspan="1" %)
71 (((
72 0.15 - 0.19
73 )))|(% colspan="1" %)(% colspan="1" %)
74 (((
75 0.30
Jussi Kenkkilä 15.1 76 )))|(% colspan="1" style="text-align:center" %)(% colspan="1" style="text-align: center;" %)
jkenkkil@helsinki_fi 12.1 77 (((
78 Widefield imaging
79 )))
80
81 == Lasers ==
82
83 4 diode laser modules  for SIM, TIRF and widefield imaging.
84
85 * 405 nn
86 * 488 nm
87 * 568 nm
88 * 640 nm
89
90 == Detectors ==
91
92 * 3 sCMOS cameras
93
94 == Software ==
95
96 ==== Image Acquisition ====
97
98 AquireSR  4.4
99
100 ==== Image Reconstruction ====
101
102 SoftWoRx 7.0
103
104 ==== **Test your own fluorophores on this optical system** ====
105
106 To do so, click on the link below, choose the right configuration and add your own fluorophore. You will see the efficiency of the system for that specific label.
107
108 [[https:~~/~~/www.fpbase.org/microscope/o44EGQpjxhqwdcehWQukx/>>url:https://www.fpbase.org/microscope/o44EGQpjxhqwdcehWQukx/||shape="rect"]]