Sample preparation

The 39 Steps: A Cautionary Tale of Quantitative 3-D Fluorescence Microscopy

http://labs.pbrc.edu/cellbiology/documents/39steps.pdf

Antibody incubation at 37°C improves fluorescent immunolabeling in free-floating thick tissue sections

https://www.future-science.com/doi/10.2144/000114524

An update: improvements in imaging perfluorocarbon-mounted plant leaves with implications for studies of plant pathology, physiology, development and cell biology

https://www.frontiersin.org/articles/10.3389/fpls.2014.00140/full