Leica TCS SP8 STED 3X CW 3D
Technical details:
MICROSCOPE:
Leica DMI8
Fully motorized inverted microscope.
SuperZ-galvanometric stage
motorized xy-stage
AFC hardware autofocus
LIS (LifeImagingServices) incubation system (THE Cube, The Box and The Brick)
OBJECTIVES:
HC PL APO 10x/0.40 CS2 (air, wd 2.56 mm)
HC PL APO 20x/0.75 IMM CORR CS2 (water, glycerol, oil, wd 0.66 mm)
HC PL APO 63x/1.20 W motCORR CS2 (water, wd 0.3 mm)
HC PL APO 93x/1.30 motCORR STED WHITE (glycerol, wd 0.3mm)
Available by request:
HC PL APO 100x/1.40 STED WHITE (oil)
The MotCORR objectives have a motorized correction collar for coverslip thickness correction
LASERS:
Red (HeNe 633 nm/12mW)
Lime (DPSS 561 nm/20mW)
Blue (Ar 458 nm/7mW, 476 nm/10mW, 488 nm/35mW, 496 nm/10mW, 514nm/35mW)
VIS AOTF
UV (diode 405 nm/50mW)
UV AOTF
BEAM SPLITTERS:
RT 15/85
Substrat
DD 458/514
TD 458/514/561
DD 488/561
TD 488/561/633
3X CW STED:
592 nm depletion laser
3D STED module
DETECTION:
Fluorescence
3 PMT
1 HyD GaAsP high sensitivity photon counting detectors
Transmitted light
1 PMT
FRAP BOOSTER:
Microscope is equipped with FRAP booster allowing FRAP experiments. However the implementation used means that spatial resolution will be lower during FRAP experiments.
AUTOFOCUS:
SP8 is equipped with adaptive focus control (AFC) that allows the microscope to maintain stable focus over long periods of time.
AFC works the best with air objectives, but water objectives can also be used. Leica application letter below explains how to operate the AFC system.
STED MANUAL:
STED SAMPLE PREPARATION:
SOFTWARE:
LAS X
3.5.2 14.1.2019
3.5.6 10.12.2019
3.5.7 19.11.2020