Zeiss LSM990 LF4D

Last modified by Mika Molin on 2026/04/27 11:19

Microscope

Axio Observer 7 with AI Sample Finder.

Objectives

EC Plan-NEOFLUAR 2.5x/0.085 WD=8.8mm
EC Plan-Neofluar 10x/0.30 WD=5.2
LD LCI Plan-Apochromat 25x/0.8 Imm Corr DIC M27 for water, silicone oil, glycerine, or oil immersion (CG=0-0.17mm) (FWD=0.57mm at CG=0.17mm) with automated water immersion
LD LCI Plan-Apochromat 40x/1.2 Imm Corr DIC FCS M27 for water, silicone oil or glycerine immersion (CG=0.15-0.19mm) (FWD=0.41mm at CG=0.17mm)
C Plan-Apochromat 63x/1.4 Oil DIC (FWD=0.14mm)

Motorized XYZstage

travel range: 130 mm x 100 mm
max. speed: 25 mm/s
resolution: 0.1 µm
reproducibility: +/- 1 µm
absolute accuracy: +/- 5 µm
Z-piezo with 500 um travel range

Light Source Viluma 7

Far Red (735/40 nm) for excitation of Cy7 and similar dyes
Red (631/33 nm) for excitation of Cy5, Alexa 631, TOTO-3 and similar dyes
Yellow (591/27) nm) for excitation of mCherry, Alexa 568, mPlum and similar dyes
Green(555/30 nm) for excitation of Cy3, TRITC, DsRed and similar dyes
Blue (469/38 nm) for excitation of eGFP, Fluo4, FITC and similar dyes
Violet (42344 nm) for excitation of eCFP, Lucifer Yellow, Alexa 430 and similar dyes
UV (385/30 nm) for excitation of DAPI, Alexa 405,Hoechst 33258 and similar dyes

Filter sets

Filter set 91 HE LED (E) for use with ZEISS LED fluorescence light sources. Suitable for fluorescent dyes like CFP, YFP and mCherry with excitation wavelengths 430, 508 and 590 nm. Contains beam splitter TBS 450 + 538 + 610 and emission filter TBP 467/24 + 555/25 + 687/145.
Filter Set 112 HE LED (E) for use with ZEISS LED fluorescence light sources. Suitable for fluorescent dyes like DAPI, FITC, DsRed, Cy5 and Cy7 with excitation wavelengths 385, 475, 555, 630 and 735 nm. Contains beam splitter PBS 405 + 493 + 575 + 654 + 761 and emission filter PBP 425/30 + 514/31 + 592/25 + 681/45 + 785/38.
Filter set 38 HE eGFP shift free (E) EX BP 470/40, BS FT 495, EM BP 525/50,
Filter set 43 HE Cy 3 shift free (E)EX BP 550/25, BS FT 570, EM
Filter set 50 Cy 5 shift free (E) EX BP 640/30, BS FT 660, EM BP 690/50

Incubator XLmulti S2 DARK Standard with temperature and CO2 control

Airyscan

Uses a 32-channel detector array instead of a single pinhole to collect more emitted light
Improves signal-to-noise and enhances resolution.
Or enables faster imaging by scanning 4 or 8 lines simultaneously.
As there is only one array, only a single channel can be recorded at a time. Imaging multiple channels using an Airy detector slows down the imaging but improved signal to noise combined to processing makes often faster to scan 1-3 channels by Airyscan than by conventional confocal.

Lightfield 4D

captures full 3D information of the sample with a single camera shot
Image taken by LightField can be combined with LSM imaging.
up to 80 volumes/ sec (single color channel)
z-range from 110µm (40x) - 1723 µm (10x)
maximal xy- resolution ~ 2µm (40x),
maximal z-resolution ~2,8µm (40x)
spectral range: excitation 385-740 nm, emission 450-800 nm

Dynamics Profiler

Module for FCS (Fluorescence Correlation Spectroscopy) measurements using a 32-channel Airy detector
Analyzes fluorescence intensity fluctuations over time, measuring diffusion rates and molecular concentration data
Enables study of molecular mobility and interactions in live samples
Enables up to 10 sequential measurement spots
Supports analysis with multiple fit models for diffusion and interaction studies
Allows export of raw Airyscan detector data for custom analysis

Confocal

Typical laser power ex fiber

405 14 mW
445 7.5 mW
514 10 mW
488 10 mW
561 10 mW
639 7.5 mW

Detectors

Transmitted light PMT detector

Spectral detector with 32 channels GaAsP PMT plus 2 channels MA-PMT

Airyscan 2 superresolution detector based on 32 concentrically arranged GaAsP detection elements. Fast acquisition with superresolution and up to 47 fps 512x512.

Camera with microlenses for Lightfield 4D.