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Olympus FV4000

Last modified by Marko Crivaro on 2026/04/27 11:00

Microscope

Fully motorized IX83 microscope frame

Objectives:

  • UPLXAPO 4x/0.16        WD 13 mm
  • UPLXAPO 10x/0.4        WD 3.1 mm
  • UPLXAPO 20x/0.80      WD 0.6 mm
  • UPLSAPO 30x/1.05 sil WD 0.80 mm                            
  • UPLSAPO 40x/1.25 sil WD 0.30 mm                
  • UPLXAPO 60x/1.42 oil WD 0.15 mm

high power LED fluorescence light source

White light LED for transmitted light viewing

Tru Focus Z-drift compensation system

cellVIVO, 37° incubator system with CO2 enrichment system

Motorized DIC prism and DIC optics for objectives 10x-60x

Software

CellSens version 3.2.1.85

Confocal scanner

Four SilVIR detectors for fluorescence. These detectors measure the absolute signal, and the system is calibrated to maintain consistent laser power levels, allowing quantification of the fluorescent signal assuming all other conditions (sample preparation, depth of signal within the sample, imaging settings) remain the same. 

Transmitted light detector

Lasers:

  • 405
  • 445
  • 488
  • 561
  • 594
  • 640

Main beam splitters

  • 405/488/561/640/730
  • 445/514/594/685
  • 405/488/594
  • 405-445/514/561
  • BS 90/10

Galvo scanner 64 X 64 – 4096 x 4096 pixels

  • Scan speed( one way) Pixel dwell time; 1 μs–1000 μs. 512 × 512 pixels; 0.92 fps, 1024 × 1024 pixels; 0.46 fps
  • Scan speed (round trip)Pixel dwell time; 0.25 μs–0.5 μs. 512 × 512 pixels; 4.0 fps, 256 × 256 pixels; 15.8 fps
  • With interlace scan 512 × 512 pixels; 15.8 fps, 256 × 256 pixels; 60.2 fps

Resonant scanner

  • max. 15 frames per second  at 1024 x 1024 pixels roundtrip
  • 30 frames per second at 1024x512 pixels
  • 438 frames per second at 1024 x 32 pixels