Microvolution

Microvolution is a pay-to-use Fiji plugin that is installed on 4D workstation that uses CUDA allowing relatively fast constrained maximum-likelihood deconvolution as long as your data is 'small enough'.  The cut-off value for small appears to be around 1 GB. 


Here we our (detailed) guide to Microvolution and the official manual. We recommend you familiarize yourself with both if you want to work with the software.  

System magnifications:

Pinhole calculator settings for Aurox Clarity Spinning disk

High sectioning physical diameter 50, pinhole spacing 50

Medium sectioning  physical diameter 100, pinhole spacing 100

Low sectioning  physical diameter 200, pinhole spacing 200