High-content screening systems
The main high-content- screening system at BIU is the ImageXpress PICO. It replaces the Thermo FisherScientific CellInsight that was disconnected in spring 2020. In addition, several wide-field microscopes are capable of automatic imaging (z-stacks, tile images and positions).
ImageXpress PICO
The Molecular Devices ImageXpress Pico is a simple high-content screening digital wide-field microscope with bright-field & multi-color fluorescence imaging with LED excitation & fixed filters. Well-plates or microscope slides can be used, and the system has environmental control for live cell experiments on well plates.
Technical information
Objectives
Magnification | Type | NA | Immersion | Correction-collar |
|---|---|---|---|---|
4x | Fluotar | 0.13 | air | - |
10x | Fluotar | 0.32 | air | - |
20x | Fluotar | 0.40 | air | - |
40x | Fluotar | 0.60 | air | Yes |
Filters
Filter | Excitation | Emission |
|---|---|---|
Dapi | 350-390 | 419-482 |
FITC | 445-485 | 509-539 |
TRITC | 509-552 | 573-613 |
Cy5 | 610-650 | 675-720 |
Transmitted light (no filter) |
|
|
Camera
Sony 5 MPixel color CMOS
Software & data
- CellReporterXpress, 5 concurrent logins possible (only 1 can control acquisition), over 20 simple analysis protocols, tiff file export possible for MetaXpress or other analysis software
- MetaXpress offline analysis software (available on Imaging Workstation)
Other features
- Hardware autofocus, additional image-based autofocus available
- Z-stack acquisition with output as maximum intensity projections or best focus slice (individual z-slice export not possible)
- Adapters for well plates (6-384 wells), 1-4 microscope slides, and live imaging chamber for well plates
- Temperature control up to 40 °C
- Active humidity control, sample chamber up to 85% humidity
- CO2 control from ambient to 15%
Info & tutorial videos
Test your own fluorophores on this optical system
To do so, click on the link below, choose the right configuration and add your own fluorophore. You will see the efficiency of the system for that specific label.