ONI Nanoimager S

Last modified by tanhuanp@helsinki_fi on 2024/01/24 07:09

ONI Nanoimager is a dSTORM, PALM, single-particle tracking, TIRF, HILO and  smFRET microscope. 

Lasers

Violet: 405 nm, 1000mW
Blue: 488 nm, 1000mW
Green: 561 nm, 1000mW
Red: 640 nm, 1000mW


Camera

sCmos camera with field of view 50µm x 80µm. 


Objective

100x 1.49 NA oil

Stage

20/20/10 mm XYZ travel range, closed-loop piezo stage with 1 nm encoder resolution


Considerations

The image is split on the camera chip so that the far red is always on the 'right' side while the other color (blue, green or red) is projected on the 'left' side. This means that if you need to imagine two channels simultaneously one of them has to be the far red channel.