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SP5 II HCA quick start.pdf

Brick gas exchanger.pdf

Technical details

MI­CRO­SCOPE:

Leica DMI6000 B 
Fully motorized inverted microscope.
SuperZ-galvanometric stage (max. moving distance 1,5 mm)
motorized xy-stage
automated DIC
LIS (LifeImagingServices) incubation system (THE Cube, The Box and The Brick)

OB­JECT­IVES:

PL Fluotar 2,5x/0,07 (air)
HC PL Fluotar 5x/0,15 (air)
HC PL APO 10x/0,4 (air)
HC PL APO 20x/0,7 CS (air)
HCX PL APO 20x/0,7 Imm Corr (water, glycerol, oil) Lbd.bl.
HCX PL APO 63x/1,2 W Corr/0,17 CS (water) Lbd.bl. with a water immersion micro dispenser

LASERS:

Red (HeNe 633 nm/12mW)
Lime (DPSS 561 nm/20mW)
Blue (Ar 458 nm/7mW, 476 nm/10mW, 488 nm/35mW, 496 nm/10mW, 514nm/35mW)
VIS AOTF

UV (diode 405 nm/50mW)
UV AOTF 
 

BEAM SPLIT­TERS:

RT 30/70
Substrat
DD 458/514
DD 488/561
RSP 500
QD 405/488/561/633
 

DETECTION:

3 PMT + 2HyD for fluorescence with adjustable detection range

1 PMT for transmitted light

FRAP BOOSTER:

Microscope is equipped with FRAP booster allowing FRAP experiments. However the implementation used means that resolution will be lost during FRAP experiments. 

SOFTWARE:

LAS AF

2.5.1; 16.6.2011

2.6.0; 17.11.2011

2.7.1; 7.1.2013

2.7.3; 9.6.2013

2.7.7; 18.7.2017

Well plates Recommended by Leica