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  • Wounding experiment
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  • Phase contrast images (time lapse) are pre-processed to find cells - not necessarily the nucleai, but any part.
  • Cell parts are divided in to "left" and "right" group by clustering the x-coordinate with k-means.
  • The cell frontiers are resolved with alpha-shape.
  • The gap area is calculated for each time point
  • A model is subjected to the data. The model expects that there is a constant decline in the area until it reaches a minimum, and stays constant at that level.
  • The time of gap closure is found.

Video of the segmentation

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