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Equipment

Evos

Inverted epifluorescence microscope with long working distance objectives for bright-field, phase-contrast, and fluorescence microscopy.
Mainly used for cells growing on plastic bottom dishes. Image acquisition through integrated camera and software, images stored directly to a memory stick.

NB! Use the USB port on the microscope. The port on the screen is not connected to it.

Technical Information and capabilities

Objectives

MagnificationObjective typeNAWorking distance (mm)Phase-contrastImmersion

4X

LPlan Fluor0.13

16.9

PhDry
10XPlan Fluor0.308.3-Dry
20XPlan Fluor0.457.1-Dry
40XPlan Fluor0.652.8-Dry

Filters

PositionLight CubeExcitation (nm)Emission (nm)
1Transmitted light--
2DAPI357/44447/60
3GFP470/22510/42
spareRFP531/44593/40
4TxRed585/29624/40

Fluorescence filters are integrated with LED light sources into 'light cubes'. The system has space for 4 such cubes or 3 + transmitted light.

Camera / Accessories

Integrated monochrome CCD camera.

An assortment of sample holders for multi-well plates, dishes, flasks, and microscope slides.


Test your own fluorophores on this optical system

To do so, click on the link below, choose the right configuration and add your own fluorophore. You will see the efficiency of the system for that specific label.

https://www.fpbase.org/microscope/KXH56NF8YtWFimM9D9Km5f/

Key Words

Wide-field

Inverted microscope

Long working distance

Zeiss Axio Im­ager 1

Motorized upright epifluorescence microscopes with transmitted light (bright field, phase contrast and dark field) and a motorized stage.

Technical Information and capabilities

Objectives

MagnificationObjective TypeNAWorking distance (mm)Phase-contrastImmersion
5xEC Plan Neofluar0.1618.5Ph1Dry
10xEC Plan Neofluar0.35.2Ph1Dry
20xPlan Apochromat0.80.55Ph2Dry
40xEC Plan Neofluar0.750.71-Dry
40xEC Plan Neofluar1.30.21-Oil
63xPlan Apochromat1.40.19-Oil
100xEC Plan Neofluar1.30.20-Oil

Filters

PositionFilterExcitationEmissionDichroic
1Transmitted light---
249 DAPI365445/50395
347HE CFP436/25480/40455
438HE GFP470/40525/50495
546HE YFP500/25535/30515
631 Alexa 568565/30620/60585
764HE mPlum587/25647/70605
850 Cy5640/30690/50660
925HE DAPI/FITC/TxRedTripleTripleTriple
10Cy7708/75810/80757

Camera

  • Hamamatsu Orca Flash 4.0 LT B&W camera for fluorescence imaging.
  • Zeiss AxioCam 105 color camera for transmitted light imaging.

Lightsource

  • HXP 120 V fluorescence light source with motorized brightness adjustment and integrated shutter.

Software

  • Zeiss Zen 2.

User manuals and quick instructions: 

How to control shutters on AxioImager

There are new Quick Start Guides available for ZEN Blue. They include the "First Steps", multidimensional imaging and import & export guides.
Some of the functions and images refer to the ZEN Lite and are different from the full version we have, which has more functionality.
There is also a video tutorial available on YouTube.

How to set up the Köhler illumination video tutorial on YouTube.

Test your own fluorophores on this optical system

To do so, click on the link below, choose the right configuration and add your own fluorophore. You will see the efficiency of the system for that specific label.

https://www.fpbase.org/microscope/5WghnCQQbmeV3sED2RmZL4/?d=hamamatsu-orca-flash40-lt

Key Words

Wide-fieldUpright microscope

Zeiss AxioImager 2 with Apotome

Motorized upright microscope for fluorescence and transmitted light imaging with a Zeiss ApoTome for optical sectioning with structured illumination.

Nearly identical to AxioImager 1, except the ApoTome and an additional Kromnigon narrow green filter (very beneficial for samples with high levels of autofluorescence visible on the green/GFP filter).

Technical Information and capabilities

Objectives

MagnificationObjective TypeNAWorking distance (mm)Phase-contrastImmersion
5xEC Plan Neofluar0.1618.5Ph1Dry
10xEC Plan Neofluar0.35.2Ph1Dry
20xPlan Apochromat0.80.55Ph2Dry
40xEC Plan Neofluar0.750.71-Dry
40xEC Plan Neofluar1.30.21-Oil
63xPlan Apochromat1.40.19-Oil
100xEC Plan Neofluar1.30.20-Oil

Filters

PositionFilterExcitationEmissionDichroic
1Transmitted light---
249 DAPI365445/50395
347HE CFP436/25480/40455
438HE GFP470/40525/50495
546HE YFP500/25535/30515
631 Alexa 568565/30620/60585
764HE mPlum587/25647/70605
850 Cy5640/30690/50660
925HE DAPI/FITC/TxRedTripleTripleTriple
10Kromnigon 488495/20524/15-

Camera

  • Hamamatsu Orca Flash 4.0 LT B&W camera for fluorescence.
  • Zeiss AxioCam 105 color camera for transmitted light.

Lightsource

  • HXP 120 V fluorescence light source with motorized brightness adjustment and integrated shutter.

Software

  • Zeiss Zen 2.

User manuals and quick instructions: 

How to control shutters on AxioImager

There are new Quick Start Guides available for ZEN Blue. They include the "First Steps", multidimensional imaging and import & export guides.
Some of the functions and images refer to the ZEN Lite and are different from the full version we have, which has more functionality.
There is also a video tutorial available on YouTube.

How to set up the Köhler illumination video tutorial on YouTube.


Test your own fluorophores on this optical system

To do so, click on the link below, choose the right configuration and add your own fluorophore. You will see the efficiency of the system for that specific label.

https://www.fpbase.org/microscope/iSNJXmZbabCsPU8tvP9Lwf/?d=hamamatsu-orca-flash40-lt

Key Words

WidefieldUpright microscopeOptical sectioning

Leica Thunder Imager Model Organism

A Leica M 205 FCA stereo microscope with computational clearing, motorized stage, and stage top incubator for live imaging. The THUNDER Imager Model Organism allows fast and easy 3D exploration of whole organisms for developmental or molecular biology research. It is the optimal instrument for studying, for example, Drosophila, C. elegans, zebrafish, plants, and mice.

Technical information

Apochromatic 16:1 zoom optics and 10x oculars

Objectives

  • 1x PlanApo stereo objective
  • 5x FluoCombi III HR objective

Light source

  • Leica LED 3 fluorescence light
  • Leica TL5000 transmitted light base

Fluorescence Filters

Please note that the microscope can handle 4 filters simultaneously (or 3 fluorescent filters + empty position for transmitted light). Always check which filters are installed.

Available filters:

FilterExcitation (nm)Emission (nm)
DAPI350/50460/50
GFP470/40525/50
mCherry560/40630/75
TXRed560/40610 LP
CY5620/60700/75

Transmitted light

  • Brightfield illumination
  • Rottermann contrast
  • Darkfield illumination

Camera

  • Leica K5 B&W sCMOS camera

Software

  • Leica LAS X
    • LAS X Navigator for motorized stage control
    • LAS X Thunder for computational clearing

Test your own fluorophores on this optical system

To do so, click on the link below, choose the right configuration and add your own fluorophore. You will see the efficiency of the system for that specific label.

https://www.fpbase.org/microscope/rH7ybAvh5EwU6E32GthEEC/

Key Words

WidefieldStereomicroscope
Optical sectioning

Leica Thunder Imager 3D Cell Culture

Motorized Leica DMi8 inverted epifluorescence microscope with  motorized stage. 

  • Leica Thunder computational clearing for 3D imaging
  • Fast multichannel imaging
  • LAS X Navigator for multipoint imaging and large area stitching

https://www.leica-microsystems.com/products/thunder-imaging-systems/p/thunder-imager-3d-live-cell/

Technical Information

Objectives

MagnificationObjectiveNAPhaseImm.WDAFCPixel sizeTheoretical lens x-y resolution at 550nmTheoretical lens z resolution at 550nm
5xN Plan0.12Ph0Dry14.0mm-1.3µm2.796µm38.194µm
10xHC PL Fluotar0.32Ph1Dry11.1mm+0.65µm1.048µm5.371µm
20xHC PL Fluotar Corr0.4Ph1Dry7.5mm+0.325µm0.839µm3.438µm
20xHC PL Apo0.8-Dry0.4mm+0.325µm0.419µm0.859µm
40xHC PL Fluotar0.8PH2Dry0.4mm+0.162µm0.419µm0.859µm
40xHC PL Apo CS21.3-Oil0.24mm+0.162µm0.258µm0.494µm

Filters

Please note that the filter cubes on this system are always used in combination with separate filter wheels for excitation and/or emission.

  • Main filter wheel:
    • Quad filter cube DFT51011 (DAPI, FITC, TRITC, Cy5)

  • Fast camera ex filter wheel
    • 440/40nm
    • 510/40nm
    • 590/50nm
    • 700/75nm
    • 100% transmission

Camera

  • Leica DFC9000GTC 4.2MPx sCMOS B&W camera.

Lightsource

  • Lumencor Spectra X LED light source with wavelengths:
    • 395nm, (filter 395/25)
    • 440nm, (filter 438/29) NO CUBE
    • 470nm, (filter 475/28)
    • 510nm, (filter 511/16) NO CUBE
    • 550nm, (filter 555/28)
    • 640nm, (filter 640/30)

Software

  • Leica LAS X 3.7.5

Maintenance

  • 40x PL APO CS2 oil removed (26.5.2023)

Nikon Ec­lipse Ti-E

Fully motorized inverted wide-field microscope for fluorescence and transmitted light imaging with a full environmental chamber.
Perfect Focus System for long time series and Lumencor Spectra X light engine
The NIS-Elements software modules allow the following imaging modes:

  • 2 channel ratio imaging
  • Fast imaging
  • High Content Screening (HCS)

Technical Information and capabilities

Objectives

MagnificationObjective typeNAWorking distance (mm)Phase-contrastImmersionPFS
4xPlan Fluor0.1316.5PhLDry-
10xPlan Fluor0.3016.0Ph1Dry
+
20xPlan Apo λ0.751.0Ph2Dry
+
40xPlan Fluor0.750.66Ph2Dry+
60xPlan Apo λ1.400.13-Oil
+
100xPlan Apo VC1.400.13-Oil
+
Available on request:
40xS Fluor1.300.22-Oil+

Filters

PositionFilterExcitationEmissionMirror
1Quad (DAPI-GFP-TxRed-Cy5)QuadQuadQuad
2LED-DAPI-A392/23447/60409
3LED-FITC-A474/27525/45495
4TxRed-4040C562/40624/40593
5LED-Cy5-A635/18680/42652
6Triple (CFP-YFP-mCherry)TripleTripleTriple

Please note that the filter cubes on this system are always used in combination with separate filter wheels for excitation and/or emission. Please refer to system settings for exact combinations.

Camera

  • Hamamatsu Orca Flash 4.0 V2 B&W camera for fluorescence.

Light source

  • Lumencor Spectra X light engine.
    • 6 solid-state light sources across the visible range
    • fast wavelength switching and power adjustment

OKO Lab Bold Line Environmental Control

Software

  • NIS-Elements Advanced Research with 6D image acquisition module and JOBS high content module.

Maintenance

  • New OKO Lab Touch control unit (10.2.2022)
  • 40× Plan Fluor 0.75NA switched to same model with Ph2 (23.11.2022)
  • Motorized stage exchanged to Nikon model (3.5.2023)
  • CO2 feed calibrated (24.5.2023)

Keywords

WidefieldInverted microscopeHigh content

Live cell

Nikon Ec­lipse Ti-E with Primo

Fully motorized inverted widefield microscope for fluorescence and transmitted light (DIC and phase contrast) imaging.

Equipped with long working distance objectives, motorized stage, and Nikon JOBS module for automated high content image acquisition.

Alveole Primo micropatterning device for fast multi-protein photopatterning.

Technical Information and capabilities

Objectives

MagnificationObjective typeNAWorking distance (mm)Phase-contrastimmersionPFSPrimo patterning compatible
4xPlan Fluor0.1317.2-Dry-yes
10xPlan Fluor0.3016.0Ph1Dry
+
20xS Plan Fluor ELWD
0.458.2-6.9-Dry
+yes
40xS Plan Fluor ELWD0.63.6-2.8Ph1Dry+
60xPlan Fluor0.850.4-0.31-Dry
-

Filters

Position

Filter

Excitation

Emission

Mirror

1

DAPI (Semrock 5060C)

377/50

447/60

409

2

GFP (Semrock 3035D-NTE)

472/30

520/35

495

3

TxRed (Semrock 4040C)

531/40

593/40

562

4

Cy5

640/20

700/75

660

5

Transmitted light

-

-

-

6

Transmitted light

-

-

-

Camera

  • Hamamatsu Orca Flash 4.0 LT B&W camera for fluorescence

Light source

  • Lumencor Sola SE II 365
    • Spectral coverage is from UV (DAPI excitation) to Red region (Cy5 excitation)
    • Precise intensity control, fast on-off switching

Software

  • NIS-Elements Advanced Research with 6D image acquisition module and JOBS high content module.


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