Equipment

Zeiss LSM 780

Motorized Zeiss Axio Observer.Z1 inverted microscope.

Technical Information and capabilities

Objectives

Laser lines

Filters

Fluorescence filters for VIS-mode

Transmitted light in Bright-field and DIC (ask for the DIC prisms if you need them).

Detectors

• 2 PMT detectors (One with extra cooling for increased sensitivity in infrared)
• GaAsP spectral detector
• Transmitted light detector (T-PMT)

Software and accessories for live cell and multi-well imaging

• Zeis ZEN 2010 software. Physiology
• FCS for GaAsP
• ROI HDR
• Image Correlation Spectroscopy analysis packages for ZEN 2010
• Definite Focus hardware for auto-focus system
• Heating Insert P S1 (temperature control)
• Incubator PM S1 (Temperature and CO₂ control)
• 40x and 63x water immersion objectives are available upon request

Keywords

Zeiss LSM 880

Zeiss inverted confocal microscope with GaAsP detector, AiryScan (Fast) detector, PicoQuant FLIM detector and a full environmental chamber.

Technical Information and capabilities

Objectives

Laser lines

 AiryScan Filters

• Plate
• BP 420-480 + BP 495-550
• BP 420-480 + BP 495-620
• BP 420-480 + LP 605
• BP 465-505 + LP 525
• BP 495-550 +LP 570
• BP 570-620 + LP 645

Main Beam Splitters

• MBS 458
• MBS 458/514
• MBS 458/461
• MBS 488
• MBS 488/561
• MBS 488/561/633
• MBS T89/R20
• Plate

Secondary Beam Splitters

• Mirror
• SBS LP 490
• SBS LP 515
• SBS LP 545
• SBS LP 635
• SBS SP 545
• SBS SP 610
• Plate

Fluorescence filters for VIS-mode

Transmitted light in Bright-field and DIC (ask for the DIC prisms if you need them).

Detectors

• 2 PMT detectors (One with extra cooling for increased sensitivity in infrared)
• 1 GaAsP detector
  Airyscan detector
  Transmitted light detector (T-PMT)

Software and accessories for live cell and multi-well imaging

• Zeis ZEN 2
• Modules: Experiment designer, Tiles & Positions, FRAP and FRET
• Definite Focus hardware autofocus system
• Scanning stage for multipoint or mosaic imaging
• Large environmental chamber
• Incubator insert PM S1 (Temperature and CO₂ control): Observation area 32 x 30 mm (oval) https://www.pecon.biz/products/product/?pid=5271%7CHeating_Insert_P_S
• 40x and 63x water immersion objectives are available upon request

Environmental Chamber Temperature Setting

The readout of the temperature probe differs slightly from the media temperature in the sample chamber. To heat the sample to +37 C, adjust the Incubator to:

• H Insert P +38.0 °C
• H Unit XL +38.2 °C

Test your own fluorophores on this optical system

To do so, click on the link below, choose the right configuration and add your own fluorophore. You will see the efficiency of the system for that specific label.

https://www.fpbase.org/microscope/fGbZLzo4KpjDLnCZqK9m6G/

Keywords

Zeiss LSM 7 MP

Upright multiphoton microscope optimized for intravital imaging of anaesthetized small laboratory animals.
The system allows acute as well chronic in vivo imaging experiments on internal (e.g. brain, kidney, liver etc.) and external organs (e.g. skin and eye).
The system can also be used for imaging fixed thick tissue samples.

Technical Information and capabilities

Objectives

Lasers

• Coherent Chameleon Vision II, tunable wavelength: 690-1010nm
• APE Chameleon Compact OPO (optical parametric oscillator), tunable wavelength 1050-1300nm

Emission filters

• Reflected PMT's: BP 500-550 (green), BP 565-610 (red)
• Transmitted PMT's: BP 500-550 (green), BP 565-610 (red)
• Transmitted light filters for Bright-field and by request DoDt contrast (inclined illumination)

Light Sources

• Widefield fluorescence and reflected light
• HXP 120 C metal halide lamp (fluorescence)
• LED-light source F1 (general-purpose light source)

Detectors

• 2 reflected light non-descanned PMT detectors with the possibility for filters
• 1 optional reflected light non-descanned nosepiece GaAsP detector without filters
• 2 transmitted light non-descanned PMT detectors with emission filters available on request
• 1 transmitted light non-descanned PMT detector available on request

Software and accessories for live cell and multi-well imaging

• ZEN 2011 software
• LinLab software for Scientifica motorized stage
• Dark microscope enclosure
• Univentor gas anesthesia system with mask, induction boxes and scavenger
• PhysioSuite hear/breath is an oxygenation monitoring system for mice and rats
• Animal's heating pad at the microscope stage to keep the animal warm during the anaesthesia
• Scientifica adjustable motorized microscope stage with a variety of intravital animal fixation adapters
• LED-light source F1 with flexible light guides for manipulations under the microscope
• Prior motorized microscope stage with multi-point scanning option available on request
• Dark environmental chamber (incubator XL) for minimization of stray light available on request
• Heating unit for temperature control of environmental chamber available on request.

Keywords

Leica SP8 CARS

Leica inverted multi-photon microscope with two HyD detectors, resonant scanner, tunable CARS laser and a full environmental chamber.

Technical Information and capabilities

Objectives

Single-photon laser lines

Multiphoton laser (CARS, TPEF, SHG)

• APE picoEmerald
• Wavelengths: 1064.5 nm and tunable 780-940 nm

Detectors

• 2 high sensitivity hybrid detectors (HyD)
• 1 PMT detector
• 2 reflected light non-descanned PMT detectors with the possibility for filters
• 2 transmitted light non-descanned PMT detectors with the possibility for filters

Software and accessories for live cell and multi-well imaging

• Full-enclosure type temperature-controlled environmental chamber for live-cell imaging and bleaching FRAP applications.
• An inverted temperature-controlled slow-flow imaging chamber available on request

Keywords

Leica TCS SP8 X

Leica TCS SP8 X with white light laser. Laser scanning confocal microscope for both fixed and live-cell imaging. Mounted on a motorized Leica inverted microscope.

Technical Information and capabilities

Objectives

Lasers

• 405 nm diode
• Supercontinuum laser, freely tunable between 470-670 nm, maximum of 8 simultaneous laser lines

Detectors, Software and accessories for live cell and multi-well imaging

• 2 high sensitivity hybrid detectors (HyD)
• 2 PMT detectors
• 1 transmitted light detector (T-PMT)

The SP8 X has a full-enclosure type temperature-controlled environmental chamber for imaging live cells. While the chamber and microscope are heating up or cooling down the light path distances are constantly changing. Because of this it is not recommended to image while the chamber temperature is changing. To keep the chamber at a steady temperature, it should be either heated (+37 °C) or at room temperature for the whole day. The first reservation created for a given day determines the temperature of the system.

Keywords

Leica Stellaris 8 FALCON / DLS

Leica Stellaris 8 inverted confocal microscope with 5 sensitive HyD detectors and tunable white-light laser excitation source.

Highlights:

• FALCON module for fast fluorescence lifetime measurement
• Digital Light-Sheet module
• TauSense detection
• Fast resonant scanner
• LASX Navigator
• Leica Lightning
• NIR excitation (up to 790nm) and detection (up to 850nm)

The LMU have made a nice video covering the capabilities of the Stellaris 8 and its FALCON module (not DLS). Definitely worth a look:

Technical information:

Microscope

• Leica DMi8 inverted microscope
• Motorized scanning stage
• AFC harware autofocus
• OKOlab full-enclosure incubator with temperature, CO₂, and humidity control

Objectives

Lasers

• 405nm diode laser
• supercontinuum laser, freely tunable between 440-790nm, maximum of 8 simultaneous laser lines

Scanner

Tandem scanner with both traditional point scanner and fast 8kHz resonant scanner.

Five sensitive HyD detectors:

• 2 HyD S - all round detector for high performance detection throughout the spectrum
• 2 HyD X - specialized for fast fluorescence lifetime imaging in the visible to the far-red
• 1 HyD R - specialized for detection above 740nm (incl. fast fluorescence lifetime)

Please note that all 5 detectors are suitable for regular confocal imaging. However, the HyD X's and HyD R are not tolerant of over exposure. If overexposed they will shut down and may be permanently damaged. You can find out more about the Leica HyD detectors and their capabilities here.

Software Leica LAS X

• 4.5.0
• 4.6.0 (5.10.2023)

Andor Dragonfly

Technical Information and capabilities

Andor Dragonfly 505

Objectives

¹⁾ Not Plan corrected

²⁾ Suitable for NIR labels. Chromatic correction 405-950nm

Lasers

• 404nm, 100mW
• 488nm, 150mW
• 561nm, 100mW
• 642nm, 110mW
• 730nm, 30mW

Filters

For visual inspection of your sample through the eyepiece

• DAPI-50606
• GFP-30309
• TRITC-B 05
• mCherry-C

For imaging

• 445 -46 nm
• 521 -38 nm
• 594 -43 nm
• 685 -47nm
• 809 -90nm
• TR-POL-DIC-FWL, Polarizer
• 445/521/594/685/809

Cameras

• Andor Zyla 4.2 sCMOS
• Andor iXon 888 U3 EMCCD

Software

• Fusion 2.0
  • ClearView-GPU online deconvolution
  • iXon SRRF-Stream real-time super-resolution
• Bitplane Imaris

Test your own fluorophores on this optical system

To do so, click on the link below, choose the right configuration and add your own fluorophore. You will see the efficiency of the system for that specific label.

https://www.fpbase.org/microscope/MHoNXQQFSXUuAowQ34PYg8/?d=andor-ixon-life-888-bv