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3i User Manual

Getting Started with SlideBook 6.pdf

Technical details

Objectives:

5x/0.16 EC Plan-Neofluar Ph1 M27
10x/0.30 EC Plan-Neofluar Ph1 WD=5.2 M27
20x/0.4 LD Plan-Neofluar Ph2 Corr WD=7.9 M27
20x/0.8 Plan-Apochromat Ph2 WD=0.55 M27
63x/1.2 W C-Apochromat Corr WD=0.28 M27
63x/1.46 Alpha Plan-Apochromat Oil Corr WD=0.10 M27

DIC slider for 63x objectives

Lasers:

Violet (solid state 405nm/100mW)
Blue (solid state 488nm/150mW)
Lime (solid state 561nm/50mW)
Red (solid state 640nm/100mW)

LED Light source:

CoolLED pE2 with 400nm/490nm/550nm/635nm set
transmitted LED

Filters:

Semrock Brightline:
LF 405/488/561/635-A
LF405-B-000
LF488-B
Chroma TIRF filter cubes:
488/561 TRF59904

 

Camera:

Andor Neo sCMOS
Specifications

Spinning disk:

Yokogawa CSU-X1 M1 5000 rpm spinning disk confocal
405/488/561/640 dichroic
Sutter LB-10W fast 10-position filter wheel:

  • quad emitter
  • 445/45
  • 525/30
  • 617/73
  • 692/40

CSUX Bybass with motorized path change


Microscope:

Zeiss Axio Observer Z1, Optovar 1x, 1,6x and 2,5x
Fully motorized inverted microscope
Motorized xy-Stage ASI MS-2000
Definite focus
The Cube & The Box The Brick CO2 mixer with active humidity control.

brick

Motorized TIRF

Vector
Point scanner for photomanipulation

mSAC
Spherical aberration correction system

Software versions
Slidebook 6.0.15 12.4.2018

How to read SlideBook SLD files in ImageJ FIJI
https://www.intelligent-imaging.com/technical-answers
(
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Microfluidistic system
CellASIC ONIX2



XY pixel size for each objective/optovar combination.

XY res (nm)   
Optovar10x20x63x
1525,6262,883,4
1.6328,5164,352,1
2.5205,3105,133,4


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